PLAAT3 Polyclonal antibody

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  WB analysis using 55209-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 55209-1-AP (PLAAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 55209-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 55209-1-AP (PLAAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLAAT3 Polyclonal Antibody for WB, ELISA

Cat No. 55209-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HRASLS3, PLA2G16

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Tested Applications

• Positive WB detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55209-1-AP targets PLAAT3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: phospholipase A2, group XVI
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: NM_007069
• Gene Symbol: PLA2G16
• Gene ID (NCBI): 11145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53816
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase A/acyltransferase 3 (PLAAT3) is a phospholipid-modifying enzyme predominantly expressed in neural and white adipose tissue (WAT).It belongs to the phospholipase A and acyltransferase (PLAAT) family which is a group of structurally related proteins that are conserved among vertebrates. In humans, the family comprises five members (PLAAT1-5), which share common domain structures, and functions as phospholipase A1/A2 and acyltransferase enzymes. Regarding acyltransferase activities, PLAATs produce N-acyl-phosphatidylethanolamines, which serve as the precursor of bioactive N-acylethanolamines (NAEs) (PMID: 40074088).