Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18018-1-AP targets PLAG1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12566 Product name: Recombinant human PLAG1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 151-500 aa of BC075047 Sequence: TCKVCLQTFESTGVLLEHLKSHAGKSSGGVKEKKHQCEHCDRRFYTRKDVRRHMVVHTGRKDFLCQYCAQRFGRKDHLTRHMKKSHNQELLKVKTEPVDFLDPFTCNVSVPIKDELLPVMSLPSSELLSKPFTNTLQLNLYNTPFQSMQSSGSAHQMITTLPLGMTCPIDMDTVHPSHHLSFKYPFSSTSYAISIPEKEQPLKGEIESYLMELQGGVPSSSQDSQASSSSKLGLDPQIGSLDDGAGDLSLSKSSISISDPLNTPALDFSQLFNFIPLNGPPYNPLSVGSLGMSYSQEEAHSSVSQLPPQTQDLQDPANTIGLGSLHSLSAAFTSSLSTSTTLPRFHQAFQ Predict reactive species |
| Full Name | pleiomorphic adenoma gene 1 |
| Calculated Molecular Weight | 500 aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC075047 |
| Gene Symbol | PLAG1 |
| Gene ID (NCBI) | 5324 |
| RRID | AB_2878482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6DJT9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PLAG1 antibody 18018-1-AP | Download protocol |
| IP protocol for PLAG1 antibody 18018-1-AP | Download protocol |
| WB protocol for PLAG1 antibody 18018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLAG1 Polyclonal antibody (Cat# 18018-1-AP) has 1 citation to date. It was cited in Neuro-Oncology Advances (2025), in a study examining a Cre-mediated copy number variant affecting a LoxP-STOP-LoxP-PLAG1-driven brain tumor model in mice. The antibody was used for Western blot. The associated research field is neuro-oncology, specifically brain tumor modeling and genetic reliability assessment.
| Species | Application | Title |
|---|---|---|
Neurooncol Adv A Cre-mediated copy number variant compromises the reliability of a LoxP-STOP-LoxP-PLAG1 driven brain tumor model. |
Reviews
What customers say
The single review for this product rates it 3 out of 5 for Western Blot. The reviewer found the antibody "fairly alright" but noted that additional washing steps may be necessary to achieve clean results. Overall, the feedback suggests the antibody performs adequately but could benefit from optimized washing conditions to reduce background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aqib (Verified Customer) (03-27-2025) | its fairly alright, may be need more washing steps for clean results
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