PLAGL1 Polyclonal antibody

PLAGL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25864-1-AP

Synonyms

Tumor suppressor ZAC, Pleiomorphic adenoma-like protein 1, LOT-1, LOT1, LOT 1

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Validation Data Gallery

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  WB analysis of MCF-7 using 25864-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25864-1-AP (PLAGL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25864-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25864-1-AP (PLAGL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 25864-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25864-1-AP (PLAGL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse heart tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25864-1-AP targets PLAGL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLAGL1 fusion protein Ag23073
• Full Name: pleiomorphic adenoma gene-like 1
• Calculated Molecular Weight: 463 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC074814
• Gene Symbol: PLAGL1
• Gene ID (NCBI): 5325
• RRID: AB_2880274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.