PLAP Recombinant monoclonal antibody, PBS Only

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• PLAP Recombinant monoclonal antibody, PBS Only (86752-3-PBS)

PLAP Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86752-3-PBS

Clone No.251261A10

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

ALPP, EC:3.1.3.1, PALP, PLAP-1

• 

  WB analysis using 86752-3-RR (same clone as 86752-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86752-3-RR (ALPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86752-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 86752-3-RR (same clone as 86752-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86752-3-RR (ALPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86752-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 86752-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86752-3-RR against Human PLAP were performed. The affinity constant is 2.06 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

86752-3-PBS targets PLAP in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3065

  Product name: Recombinant human ALPP protein (C-rFc)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 23-506 aa of NM_001632.4

  Sequence: IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD

• Full Name: alkaline phosphatase, placental (Regan isozyme)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_001632.4
• Gene Symbol: PLAP
• Gene ID (NCBI): 250
• RRID: AB_3745102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05187
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.