PLCB3 Recombinant antibody

• 

  WB analysis using 85786-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85786-1-RR (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PLCB3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85786-1-RR

Clone No.243045B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, MCF-7 cells, U2OS cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85786-1-RR targets PLCB3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: phospholipase C, beta 3 (phosphatidylinositol-specific)
• Calculated Molecular Weight: 1234 aa, 139 kDa
• Observed Molecular Weight: 139-150 kDa
• GenBank Accession Number: BC142681
• Gene Symbol: PLCB3
• Gene ID (NCBI): 5331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01970
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.