Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells, L02 cells, SK-N-SH cells |
| Positive IHC detected in | human stomach tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17327-1-AP targets PLD3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11306 Product name: Recombinant human PLD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 144-490 aa of BC036327 Sequence: GEEVLRQLQTLAPKGVNVRIAVSKPSGPQPQADLQALLQSGAQVRMVDMQKLTHGVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYVAVMNYLPTLEFSHPHRFWPAIDDGLRRATYERGVKVRLLISCWGHSEPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADEAQARIPYARVNHNKYMVTERATYIGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYSHDLDTSADSVGNACRLL Predict reactive species |
| Full Name | phospholipase D family, member 3 |
| Calculated Molecular Weight | 490 aa, 55 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC036327 |
| Gene Symbol | PLD3 |
| Gene ID (NCBI) | 23646 |
| RRID | AB_2878383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IV08 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PLD3 antibody 17327-1-AP | Download protocol |
| IHC protocol for PLD3 antibody 17327-1-AP | Download protocol |
| WB protocol for PLD3 antibody 17327-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLD3 antibody (Cat# 17327-1-AP) has 4 total citations published in Cell Death Discovery, ACS Applied Materials & Interfaces, Human Molecular Genetics, and International Journal of Cardiology: Heart and Vascular. The research fields span lipid metabolism and therapy-induced senescence, Alzheimer's disease modeling, Parkinson's disease (CHCHD2 mutation), and atherosclerosis involving foam cell formation and inflammatory responses. Applications include WB, IF, and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Discov A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence. | ||
ACS Appl Mater Interfaces FRET Nanosensor Based on DNA Tetrahedron for Visualizing PLD3 Fluctuation in Mouse Models of Alzheimer's Disease | ||
Hum Mol Genet Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation. | ||
Int J Cardiol Heart Vasc Macrophage Phospholipase D3 promotes atherosclerosis via exacerbating foam cell formation and inducing inflammatory responses.
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