PLEK Polyclonal antibody

• Featured Product
• KD/KO Validated

PLEK Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC

Conjugate

Unconjugated

Publications

3

Cat no : 12506-1-AP

Synonyms

P47, Platelet 47 kDa protein, pleckstrin, PLEK

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Validation Data Gallery

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  WB analysis of U-937 using 12506-1-AP

  WB result of PLEK antibody (12506-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLEK transfected U-937 cells.

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  WB analysis of U-937 using 12506-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12506-1-AP (PLEK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 12506-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 12506-1-AP (PLEK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 12506-1-AP

  IP result of anti-PLEK (IP:12506-1-AP, 3ug; Detection:12506-1-AP 1:1000) with U-937 cells lysate 3000ug.

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  IHC staining of human lung cancer using 12506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12506-1-AP (PLEK antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: U-937 cells, rat spleen tissue
• Positive IP detected in: U-937 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

12506-1-AP targets PLEK in WB, IP, IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLEK fusion protein Ag3187
• Full Name: pleckstrin
• Calculated Molecular Weight: 350 aa, 40 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: BC018549
• Gene Symbol: PLEK
• Gene ID (NCBI): 5341
• RRID: AB_2283809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

Publications

Species	Application	Title
human	WB	Mol Med; DMAG, a novel countermeasure for the treatment of thrombocytopenia.; Authors - Jing Lin
human,mouse	WB	Front Neurosci; MiR-409-5p as a Regulator of Neurite Growth Is Down Regulated in APP/PS1 Murine Model of Alzheimer's Disease.; Authors - Jing Guo ; KD Validated
human	WB	mSphere; Ehrlichia chaffeensis TRP75 Interacts with Host Cell Targets Involved in Homeostasis, Cytoskeleton Organization, and Apoptosis Regulation To Promote Infection.; Authors - Tian Luo