PLEK2 Recombinant antibody

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  WB analysis using 85619-4-RR

  HT-29, U2OS, PC-3, Caco-2 (positive) and Ramos (negative) cells were subjected to SDS PAGE followed by western blot with 85619-4-RR (PLEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85619-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85619-4-RR against Human PLEK2 were performed. The affinity constant is below 1 pM.

PLEK2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85619-4-RR

Clone No.242987B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Pleckstrin 2, Pleckstrin-2

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Tested Applications

• Positive WB detected in: HT-29 cells, U2OS cells, PC-3 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85619-4-RR targets PLEK2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PLEK2 fusion protein Ag2297
• Full Name: pleckstrin 2
• Calculated Molecular Weight: 353 aa, 40 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC001226
• Gene Symbol: PLEK2
• Gene ID (NCBI): 26499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NYT0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLEK2 belongs to the Pleckstrin homology domain family and is a 40-kDa protein containing the prototypic PH domains at its amino and carboxyl termini(PMID: 10419454). PLEK2 associates with membrane-bound phosphatidylinositols generated by phosphatidylinositol 3-kinase. PLEK2 interacts with the actin cytoskeleton to induce cell spreading.