Tested Applications
| Positive WB detected in | COLO 320 cells, Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11292-1-AP targets PLEKHA3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1851 Product name: Recombinant human PLEKHA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC044567 Sequence: MEGVLYKWTNYLTGWQPRWFVLDNGILSYYDSQDDVCKGSKGSIKMAVCEIKVHSADNTRMELIIPGEQHFYMKAVNAAERQRWLVALGSSKACLTDTRTKKEKEISETSESLKTKMSELRLYCDLLMQQVHTIQEFVHHDENHSSPSAENMNEASSLLSATCNTFITTLEECVKIANAKFKPEMFQLHHPDPLVSPVSPSPVQMMKRSVSHPGSCSSERSSHSIKEPVSTLHRLSQRRRRTYSDTDSCSDIPLEDPDRPVHCSKNTLNGDLASATIPEESRLMAKKQSESEDTLPSFSS Predict reactive species |
| Full Name | pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 3 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC044567 |
| Gene Symbol | PLEKHA3 |
| Gene ID (NCBI) | 65977 |
| RRID | AB_10637846 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HB20 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHA3 antibody 11292-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLEKHA3 Polyclonal antibody (Cat# 11292-1-AP) has 1 total citation. The cited publication appeared in bioRxiv (2024) and investigated spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists, placing it in the research fields of inflammasome biology and proteomics. The antibody was applied in Western Blot (WB) using human samples.
| Species | Application | Title |
|---|---|---|

