PLEKHF2 Polyclonal antibody

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  WB analysis using 25424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25424-1-AP (PLEKHF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brown adipose using 25424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 25424-1-AP (PLEKHF2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brown adipose using 25424-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 25424-1-AP (PLEKHF2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brown adipose using 25424-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 25424-1-AP (PLEKHF2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brown adipose using 25424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using PLEKHF2 antibody (25424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brown adipose using 25424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using PLEKHF2 antibody (25424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLEKHF2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 25424-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Phafin 2, PHAFIN2, ZFYVE18

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: rat brown adipose tissue, mouse brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brown adipose tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25424-1-AP targets PLEKHF2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22285

  Product name: Recombinant human PLEKHF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 186-249 aa of BC011806

  Sequence: CSEKRFLLPSQSSKPVRICDFCYDLLSAGDMATCQPARSDSYSQSLKSPLNDMSDDDDDDDSSD

• Full Name: pleckstrin homology domain containing, family F (with FYVE domain) member 2
• Calculated Molecular Weight: 249 aa, 28 kDa
• Observed Molecular Weight: 22-27 kDa
• GenBank Accession Number: BC011806
• Gene Symbol: PLEKHF2
• Gene ID (NCBI): 79666
• RRID: AB_3669474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H8W4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phafin2 is a lysosomal protein consisting of 249 amino acids with a unique structure containing both an N-terminal PH (pleckstrin homology) domain and C terminal FYVE (Fab 1, YOTB, Vac 1, and EEA1) domain.