PLEKHG4 Polyclonal antibody

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• PLEKHG4 Polyclonal antibody (17156-1-AP)

PLEKHG4 Polyclonal Antibody for WB, ELISA

Cat No. 17156-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

PH domain-containing family G member 4, Pleckstrin homology domain-containing family G member 4, PRTPHN1, Puratrophin 1, Puratrophin-1

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  WB analysis using 17156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17156-1-AP (PLEKHG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells, SH-SY5Y cells, SH-N-SH cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17156-1-AP targets PLEKHG4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10328

  Product name: Recombinant human PLEKHG4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC082974

  Sequence: MHVKDPGPPRPPAGATQDEELQGSPLSRKFQLPPAADESGDAQRGTVESSSVLSEGPGPSGVESLLCPMSSHLSLAQGESDTPGVGLVGDPGPSRAMPSGLSPGALDSDPVGLGDPLSEISKLLEAAPSGSGLPKPADCLLAQDLCWELLASGMATLPGTRDVQGRAVLLLCAHSPAWLQSECSSQELIRLLLYLRSIPRPEVQALGLTVLVDARICAPSSSLFSGLSQLQEAAPGAVYQVLLVGSTLLKEVPSGLQLEQLPSQSLLTHIPTAGLPTSLGGGLPYCHQAWLDFRRRLEALLQNCQAACALLQGAIESVKAVPQPMEPGEVGQLLQQTEVLMQQVLDSPWL

• Full Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 4
• Calculated Molecular Weight: 1151 aa, 126 kDa
• Observed Molecular Weight: 131 kDa
• GenBank Accession Number: BC082974
• Gene Symbol: PLEKHG4
• Gene ID (NCBI): 25894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q58EX7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PLEKHG4 protein (full name: Pleckstrin Homology and RhoGEF Domain Containing G4), also known as Puratrophin-1, belongs to the Rho guanine nucleotide exchange factor (RhoGEF) family containing Dbl homology (DH) domains and pleckstrin homology (PH) domains. Its core function is to act as a GEF, catalyzing the conversion of small GTPases of the Rho family from the inactive GDP-bound state to the active GTP-bound state, thereby playing a key role in intracellular signal transduction and Golgi cytoskeletal dynamics.

Publications

What published studies show

The PLEKHG4 Polyclonal antibody (Cat# 17156-1-AP) has a total of 1 citation. The citation appears in the journal Apoptosis (2023), where PLEKHG4 was studied for its role in activating RhoGTPases and promoting malignant phenotypes in thyroid cancer. The associated research field is oncology, specifically thyroid cancer biology and Rho GTPase signaling pathways.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Apoptosis; Pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4) leads to the activation of RhoGTPases promoting the malignant phenotypes of thyroid cancer; Authors - Qingling Yuan