PLEKHG5 Polyclonal antibody

• 

  WB analysis of mouse heart using 19830-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19830-1-AP (PLEKHG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 19830-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19830-1-AP (PLEKHG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PLEKHG5 Polyclonal Antibody for WB, ELISA

Cat No. 19830-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DSMA4, GEF720, KIAA0720, PLEKHG5, RP4 650H14.3

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Tested Applications

• Positive WB detected in: mouse heart tissue, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

19830-1-AP targets PLEKHG5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11725

  Product name: Recombinant human PLEKHG5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 597-930 aa of BC015231

  Sequence: KDSKMDVYCFLFTDLLLVTKAVKKAERTRVIRPPLLVDKIVCRELRDPGSFLLIYLNEFHSAVGAYTFQASGQALCRGWVDTIYNAQNQLQQLRAQEPPGSQQPLQSLEEEEDEQEEEEEEEEEEEEGEDSGTSAASSPTIMRKSSGSPDSQHCASDGSTETLAMVVVEPGDTLSSPEFDSGPFSSQSDETSLSTTASSATPTSELLPLGPVDGRSCSMDSAYGTLSPTSLQDFVAPGPMAELVPRAPESPRVPSPPPSPRLRRRTPVQLLSCPPHLLKSKSEASLLQLLAGAGTHGTPSAPSRSLSELCLAVPAPAQEADPGPALPNQDHPAA

• Full Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 5
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 105-125 kDa
• GenBank Accession Number: BC015231
• Gene Symbol: PLEKHG5
• Gene ID (NCBI): 57449
• RRID: AB_10858324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94827
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Sci Signal; RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms.; Authors - Junning Yang
mouse	WB	J Neurosci; NG2 Regulates Directional Migration of Oligodendrocyte Precursor Cells via Rho GTPases and Polarity Complex Proteins.; Authors - Binamé Fabien F
mouse	WB	FASEB J; Characterization and computational simulation of human Syx, a RhoGEF implicated in glioblastoma; Authors - Ryan J Boyd