Tested Applications
Positive WB detected in | A431 cells, HT-29 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
33028-1-AP targets PLEKHN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PLEKHN1 fusion protein Ag38741 Predict reactive species |
Full Name | pleckstrin homology domain containing, family N member 1 |
Calculated Molecular Weight | 663 aa, 72 kDa |
Observed Molecular Weight | 66-71 kDa |
GenBank Accession Number | BC101386 |
Gene Symbol | PLEKHN1 |
Gene ID (NCBI) | 84069 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | Q494U1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLEKHN1 (Pleckstrin Homology Domain Containing N1) is a protein-coding gene located on chromosome 1. It plays a crucial role in various cellular processes, including apoptosis and mRNA stability regulation. PLEKHN1 has been identified as a pro-apoptotic protein that enhances Bax-Bak hetero-oligomerization via interaction with Bid in colon cancer cells. (PMID: 29531808)
Protocols
Product Specific Protocols | |
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WB protocol for PLEKHN1 antibody 33028-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |