PLEKHO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLEKHO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 24883-1-AP

Synonyms

C Jun binding protein, CK2 interacting protein 1, CKIP 1, CKIP1, CKIP-1, HQ0024c, JBP, OC120, PLEKHO1

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Validation Data Gallery

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  WB analysis using 24883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24883-1-AP (PLEKHO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 24883-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24883-1-AP (PLEKHO1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24883-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24883-1-AP (PLEKHO1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

24883-1-AP targets PLEKHO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLEKHO1 fusion protein Ag19309
• Full Name: pleckstrin homology domain containing, family O member 1
• Calculated Molecular Weight: 409 aa, 46 kDa
• Observed Molecular Weight: 46 kDa, 90 kDa
• GenBank Accession Number: BC010149
• Gene Symbol: PLEKHO1
• Gene ID (NCBI): 51177
• RRID: AB_2879778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLEKHO1, also named as CK2 interacting protein 1 or OC120, is a 409 amino acid protein, which contains one PH domain. PLEKHO1 Predominantly localizes to the plasma membrane. PLEKHO1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). Catalog#24883-1-AP recognizes 46 kDa and 90 kDa homodimer band.

Publications

Species	Application	Title
mouse	WB	Bioact Mater; Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy; Authors - Xin Huang
mouse	WB,IHC	J Dent Res; Ckip-1 Mediates P. gingivalis-Suppressed Cementoblast Mineralization.; Authors - X Huang ; KD Validated
mouse	WB	Front Physiol; Myocardial CKIP-1 Overexpression Protects from Simulated Microgravity-Induced Cardiac Remodeling.; Authors - Shukuan Ling
mouse	WB	Front Physiol; Casein Kinase-2 Interacting Protein-1 Regulates Physiological Cardiac Hypertrophy via Inhibition of Histone Deacetylase 4 Phosphorylation.; Authors - Yinlong Zhao
rat	WB	Front Pharmacol; Exploratory study of sea buckthorn enhancing QiangGuYin efficacy by inhibiting CKIP-1 and Notum activating the Wnt/β-catenin signaling pathway and analysis of active ingredients by molecular docking; Authors - Yi-Feng Yuan
mouse	WB	J Oral Microbiol; CKIP-1 mediates P. gingivalis-suppressed osteogenic/cementogenic differentiation of periodontal ligament cells partially via p38 signaling pathway; Authors - Xin Huang