PLEKHO2 Polyclonal antibody

PLEKHO2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21356-1-AP

Synonyms

PLEKHO2, PLEKHQ1, PP1628, pp9099

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Validation Data Gallery

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  WB analysis of HeLa using 21356-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21356-1-AP (PLEKHO2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse thymus using 21356-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21356-1-AP (PLEKHO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 21356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21356-1-AP (PLEKHO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 21356-1-AP

  Immunofluorescent analysis of A431 cells using 21356-1-AP (PLEKHO2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse thymus tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 21356-1-AP is PLEKHO2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLEKHO2 fusion protein Ag15786
• Full Name: pleckstrin homology domain containing, family O member 2
• Calculated Molecular Weight: 490 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC008744
• Gene Symbol: PLEKHO2
• Gene ID (NCBI): 80301
• RRID: AB_2878846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pleckstrin homology domain-containing proteins family O member 2 (PLEKHO2)'s function remains largely unknown. Genetic polymorphisms of PLEKHO2 contribute to diabetic retinopathy (DR) development. Functional prediction analysis strengthened the likelihood of participation of PLEKHO2 in the development of DR (Eric C. Han et al., 2012).

Publications

Species	Application	Title
human	IHC	Front Immunol; Exploring the immune microenvironment of osteosarcoma through T cell exhaustion-associated gene expression: a study on prognosis prediction; Authors - Junchao Zhu