Perilipin 1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83905-4-RR (same clone as 83905-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83905-4-RR (PLIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83905-4-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 83905-4-RR (same clone as 83905-4-PBS)

  IP result of anti-Perilipin 1 (IP:83905-4-RR, 4ug; Detection:83905-4-RR 1:4000) with MCF-7 cells lysate 1240 ug. This data was developed using the same antibody clone with 83905-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83905-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83905-4-RR against Human Perilipin 1 were performed. The affinity constant is 0.617 nM.

Perilipin 1 Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 83905-4-PBS

Clone No.240994H1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

PLIN, 240994H1, PERI, PLIN1

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Product Information

83905-4-PBS targets Perilipin 1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26426

  Product name: Recombinant human PLIN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 91-200 aa of NM_001145311

  Sequence: MACRGLDHLEEKIPALQYPPEKIASELKDTISTRLRSARNSISVPIASTSDKVLGAALAGCELAWGVARDTAEFAANTRAGRLASGGADLALGSIEKVVEYLLPPDKEESA

• Full Name: perilipin
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_001145311
• Gene Symbol: Perilipin 1
• Gene ID (NCBI): 5346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O60240
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).