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  • KD/KO Validated

PLK3 Polyclonal antibody

PLK3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10977-1-AP

Synonyms

CNK, FGF inducible kinase, FNK, PLK 3, PLK3, Polo like kinase 3, PRK, Proliferation related kinase



Tested Applications

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10977-1-AP targets PLK3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK3 fusion protein Ag1431
Full Name polo-like kinase 3 (Drosophila)
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC013899
Gene Symbol PLK3
Gene ID (NCBI) 1263
RRIDAB_2299937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53(PMID:12548019).

Protocols

Product Specific Protocols
IHC protocol for PLK3 antibody 10977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Hepatology

Polo-like kinase 2 is a mediator of hedgehog survival signaling in cholangiocarcinoma.

Authors - Fingas Christian D CD
  • KD Validated
humanWB,IF

Mol Cell Biol

Plk3 interacts with and specifically phosphorylates VRK1 in Ser342, a downstream target in a pathway that induces Golgi fragmentation.

Authors - López-Sánchez Inmaculada I
humanIHC

Liver Int

Polo-like kinase 3 is associated with improved overall survival in cholangiocarcinoma.

Authors - Benjamin Juntermanns
humanIHC

Clin Experiment Ophthalmol

Prognostic significance of polo-like kinases in retinoblastoma: correlation with patient outcome, clinical and histopathological parameters.

Authors - Lata Singh
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu

Mol Cancer

LINC00115 promotes chemoresistant breast cancer stem-like cell stemness and metastasis through SETDB1/PLK3/HIF1α signaling

Authors - Fei Luo