Plasmolipin Polyclonal antibody

Plasmolipin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14868-1-AP

Synonyms

Plasma membrane proteolipid, Plasmolipin, PLLP, PMLP, TM4SF11

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Validation Data Gallery

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  WB analysis of human testis using 14868-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14868-1-AP (Plasmolipin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14868-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14868-1-AP (Plasmolipin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 14868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14868-1-AP (Plasmolipin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14868-1-AP (Plasmolipin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14868-1-AP (Plasmolipin antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14868-1-AP (Plasmolipin antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue, rat brain tissue
• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1500
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14868-1-AP targets Plasmolipin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Plasmolipin fusion protein Ag6425
• Full Name: plasma membrane proteolipid (plasmolipin)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC002760
• Gene Symbol: PLLP
• Gene ID (NCBI): 51090
• RRID: AB_2284162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.