Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14868-1-AP targets Plasmolipin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6425 Product name: Recombinant human PLLP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-182 aa of BC002760 Sequence: MAEFPSKVSTRTSSPAQGAEASVSALRPDLGFVRSRLGALMLLQLVLGLLVWALIADTPYHLYPAYGWVMFVAVFLWLVTIVLFNLYLFQLHMKLYMVPWPLVLMIFNISATVLYITAFIACSAAVDLTSLRGTRPYNQRAAASFFACLVMIAYGVSAFFSYQAWRGVGSNAATSQMAGGYA Predict reactive species |
| Full Name | plasma membrane proteolipid (plasmolipin) |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC002760 |
| Gene Symbol | PLLP |
| Gene ID (NCBI) | 51090 |
| RRID | AB_2284162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y342 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Plasmolipin antibody 14868-1-AP | Download protocol |
| WB protocol for Plasmolipin antibody 14868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Plasmolipin Polyclonal antibody (Cat# 14868-1-AP) has 1 citation. It appears in the Journal of Biological Chemistry (IF 4.1), published in 2026. The cited study investigates how PLLP inhibits WT p53 gastric cancer progression by reducing p53 protein ubiquitination through binding to TRIM59. The research field encompasses gastric cancer biology, p53-mediated tumor suppression, and protein ubiquitination pathways. The antibody was used in WB and IHC applications on human samples.
| Species | Application | Title |
|---|---|---|
J Biol Chem PLLP inhibits the progression of WT p53 gastric cancer by reducing p53 protein ubiquitination by binding to TRIM59.
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