Phospholamban Polyclonal antibody

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  WB analysis using 15873-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15873-1-AP (PLN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 15873-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15873-1-AP (Phospholamban antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15873-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15873-1-AP (Phospholamban antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Phospholamban Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15873-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PLN, PLB, CMD1P

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15873-1-AP targets Phospholamban in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Phospholamban fusion protein Ag8632
• Full Name: phospholamban
• Calculated Molecular Weight: 52 aa, 6 kDa
• Observed Molecular Weight: 10 kDa, 25 kDa
• GenBank Accession Number: BC005269
• Gene Symbol: PLN
• Gene ID (NCBI): 5350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P26678
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholamban (PLN) is a 52-amino-acid transmembrane protein found in the sarcoplasmic reticulum (SR) of cardiac muscle cells. It has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. Phospholamban has a homopentamer form(25-27 kDa).