PLOD2 Recombinant monoclonal antibody, PBS Only

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• PLOD2 Recombinant monoclonal antibody, PBS Only (88222-2-PBS)

PLOD2 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 88222-2-PBS

Clone No.260421A7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

EC:1.14.11.4, Lysyl hydroxylase 2, PLOD2/LH2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

• 

  WB analysis using 88222-2-RR (same clone as 88222-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 88222-2-RR (PLOD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88222-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 88222-2-RR (same clone as 88222-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 88222-2-RR (PLOD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88222-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 88222-2-RR (same clone as 88222-2-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 88222-2-RR (PLOD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88222-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

88222-2-PBS targets PLOD2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15614

  Product name: Recombinant human PLOD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 459-540 aa of BC037169

  Sequence: PYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTA

• Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
• Calculated Molecular Weight: 758 aa, 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC037169
• Gene Symbol: PLOD2
• Gene ID (NCBI): 5352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00469
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.