PLOD3 Polyclonal antibody

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• PLOD3 Polyclonal antibody (11027-1-AP)

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PLOD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11027-1-AP Copied!

Publications (38)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Galactosylhydroxylysine-glucosyltransferase, LH3, Lysyl hydroxylase 3, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Procollagen galactosyltransferase

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  WB analysis using 11027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis using 11027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of mouse placenta using 11027-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HepG2 using 11027-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of A549 using 11027-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of PC-3 using 11027-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HeLa using 11027-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of human placenta using 11027-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HepG2 using 11027-1-AP

  IP result of anti-PLOD3 (IP:11027-1-AP, 3ug; Detection:11027-1-AP 1:500) with HepG2 cells lysate 6000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human pancreas cancer using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human pancreas cancer using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11027-1-AP (PLOD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11027-1-AP (PLOD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IF Staining of HepG2 using 11027-1-AP

  Immunofluorescent analysis of HepG2 cells using 11027-1-AP (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 11027-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PLOD3 antibody (11027-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, PC-3 cells, HepG2 cells, HeLa cells, human placenta tissue, mouse placenta tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue, human pancreas tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11027-1-AP targets PLOD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1480

  Product name: Recombinant human PLOD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 339-738 aa of BC011674

  Sequence: HEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP

• Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
• Calculated Molecular Weight: 738 aa, 85 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC011674
• Gene Symbol: PLOD3
• Gene ID (NCBI): 8985
• RRID: AB_2165781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60568
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Publications

What published studies show

The anti-PLOD3 antibody (Cat# 11027-1-AP) has 38 citations published in journals including Nature Communications, Science Advances, Cell Death & Disease, Matrix Biology, Journal of Biological Chemistry, PLoS Genetics, Glycobiology, and International Journal of Molecular Sciences. Research fields span collagen post-translational modification, extracellular matrix biology, connective tissue disorders (osteogenesis imperfecta, epidermolysis bullosa), cancer (lung, gastric, cervical, colon, breast), fibrosis, and glycosylation.

Automatically generated from the 38 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Nat Commun; The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes; Authors - Shuangcheng Alivia Wu
		Nat Commun; Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.; Authors - Luigi Scietti
human	WB,IF	Nat Commun; Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.; Authors - Blerida Banushi
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
human	WB,IP,IHC,IF	Cell Death Dis; PLOD3 promotes lung metastasis via regulation of STAT3.; Authors - Jeong-Hwa Baek ; KD Validated
human	WB,IF	Cell Death Dis; PLOD3 suppression exerts an anti-tumor effect on human lung cancer cells by modulating the PKC-delta signaling pathway.; Authors - Jeong-Hwa Baek ; KD Validated