PLS3 Polyclonal antibody

PLS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 12917-1-AP

Synonyms

T-plastin, T-fimbrin, T plastin, Plastin-3, Plastin 3

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Validation Data Gallery

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  WB analysis using 12917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12917-1-AP (PLS3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12917-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12917-1-AP (PLS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 12917-1-AP

  IP result of anti-PLS3 (IP:12917-1-AP, 4ug; Detection:12917-1-AP 1:500) with A431 cells lysate 2640 ug.

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  IHC staining of human skin cancer using 12917-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12917-1-AP (PLS3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12917-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12917-1-AP (PLS3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 12917-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using PLS3 antibody (12917-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 12917-1-AP

  Immunofluorescent analysis of A431 cells using 12917-1-AP (PLS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12917-1-AP targets PLS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLS3 fusion protein Ag3574
• Full Name: plastin 3 (T isoform)
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC039049
• Gene Symbol: PLS3
• Gene ID (NCBI): 5358
• RRID: AB_2877892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLS3, also named T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. L-plastin plays an important role in leukocyte function, and T-plastin is possibly involved in DNA repair. Both T- and L-plastin are implicated in several diseases, and L-plastin is considered to be a valuable marker for cancer (PMID: 15960882).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development.; Authors - Jasmin Morandell

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gaurav (Verified Customer) (02-06-2024)

Excellent antibody with expected molecular weight at right position. Works very well in knock out samples of Smooth Muscle Cells. Works even better at room temperature also. Very stable antibody.

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Aorta, Placenta

PLS3 Antibody Western Blot,Immunoprecipitation,Cell culture validation (1:5000 dilution) in Aorta, Placenta (Cat no:12917-1-AP)