PLS3 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86323-1-RR (same clone as 86323-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86323-1-RR (PLS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86323-1-RR (same clone as 86323-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86323-1-RR (PLS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 86323-1-RR (same clone as 86323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86323-1-RR (PLS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 86323-1-RR (same clone as 86323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86323-1-RR (PLS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86323-1-RR (same clone as 86323-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLS3 antibody (86323-1-RR, Clone: 250908B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86323-1-PBS in a different storage buffer formulation.

PLS3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86323-1-PBS

Clone No.250908B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Plastin 3, Plastin-3, T plastin, T-fimbrin, T-plastin

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Product Information

86323-1-PBS targets PLS3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: plastin 3 (T isoform)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_005032
• Gene Symbol: PLS3
• Gene ID (NCBI): 5358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13797
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLS3, also named T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. L-plastin plays an important role in leukocyte function, and T-plastin is possibly involved in DNA repair. Both T- and L-plastin are implicated in several diseases, and L-plastin is considered to be a valuable marker for cancer (PMID: 15960882).