PLSCR1 Recombinant monoclonal antibody

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  WB analysis using 84888-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84888-4-RR (PLSCR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84888-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84888-4-RR against Human PLSCR1 were performed. The affinity constant is 0.377 nM.

PLSCR1 Recombinant Antibody for WB, ELISA

Cat No. 84888-4-RR

Clone No.242372B2

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

phospholipid scramblase 1, MMTRA1B, Mg(2+)-dependent nuclease, EC:3.1.-.-, Ca(2+)-dependent phospholipid scramblase 1

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, A549 cells, MCF-7 cells, Jurkat cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84888-4-RR targets PLSCR1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2172

  Product name: Recombinant human PLSCR1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-318 aa of BC021100

  Sequence: MDKQNSQMNASHPETNLPVGYPPQYPPTAFQGPPGYSGYPGPQVSYPPPPAGHSGPGPAGFPVPNQPVYNQPVYNQPVGAAGVPWMPAPQPPLNCPPGLEYLSQIDQILIHQQIELLEVLTGFETNNKYEIKNSFGQRVYFAAEDTDCCTRNCCGPSRPFTLRIIDNMGQEVITLERPLRCSSCCCPCCLQEIEIQAPPGVPIGYVIQTWHPCLPKFTIQNEKREDVLKISGPCVVCSCCGDVDFEIKSLDEQCVVGKISKHWTGILREAFTDADNFGIQFPLDLDVKMKAVMIGACFLIDFMFFESTGSQEQKSGVW

• Full Name: phospholipid scramblase 1
• Calculated Molecular Weight: 318 aa, 35 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC021100
• Gene Symbol: PLSCR1
• Gene ID (NCBI): 5359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15162
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Olga (Verified Customer) (10-03-2025)

For those who, like me, did not enter into the details of the epitope, this antibody IS NOT SUITABLE FOR MOUSE PLSCR1 detection! Despite that it detects the only clear band of the right size in mouse liver and brain – this is not PLSCR1! – as at the same time it does not recognize recombinant overexpressed PLSCR1.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissues and cell line: liver, forebrain, Neuro2A

PLSCR1 Antibody Western Blot validation (1:1000 dilution) in mouse tissues and cell line: liver, forebrain, Neuro2A (Cat no:84888-4-RR)