PLTP Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84592-1-RR (same clone as 84592-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84592-1-RR (PLTP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84592-1-RR (PLTP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84592-1-RR (same clone as 84592-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PLTP antibody (84592-1-RR, Clone: 241583F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84592-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84592-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84592-1-RR against Human PLTP were performed. The affinity constant is below 1 pM.

PLTP Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 84592-1-PBS

Clone No.241583F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

241583F3, Lipid transfer protein II, Phospholipid transfer protein

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Product Information

84592-1-PBS targets PLTP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8171

  Product name: Recombinant human PLTP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 163-441 aa of BC005045

  Sequence: IDYSLMKDPVASTSNLDMDFRGAFFPLTERNWSLPNRAVEPQLQEEERMVYVAFSEFFFDSAMESYFRAGALQLLLVGDKVPHDLDMLLRATYFGSIVLLSPAVIDSPLKLELRVLAPPRCTIKPSGTTISVTASVTIALVPPDQPEVQLSSMTMDARLSAKMALRGKALRTQLDLRRFRIYSNHSALESLALIPLQAPLKTMLQIGVMPMLNERTWRGVQIPLPEGINFVHEVVTNHAGFLTIGADLHFAKGLREVIEKNRPADVRASTAPTPSTAAV

• Full Name: phospholipid transfer protein
• Calculated Molecular Weight: 493 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC005045
• Gene Symbol: PLTP
• Gene ID (NCBI): 5360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55058
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLTP (phospholipid transfer protein) belongs to the family of lipid-binding and lipid-transfer proteins. PLTP is involved in the distribution and metabolism of high-density lipoprotein ( HDL ) and reverse cholesterol transfer ( RCT ). In plasma, PLTP catalyzes the transfer of phospholipids ( mainly phosphatidylcholine ) between lipoproteins (PMID: 29558025). PLTP has a predicted protein molecular mass of approximately 55 kDa, and molecular weights of 70-80 kDa can also be detected in some literature (PMID: 26160914; 36312255; 29680823; 15342679; 7654777).