PLUNC Polyclonal antibody

PLUNC Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10413-1-AP

Synonyms

BPIFA1, LPLUNC3, Lung specific protein X, LUNX, NASG, PLUNC, Protein Plunc, SPLUNC1, SPURT, Von Ebner protein Hl

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Validation Data Gallery

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  WB analysis of mouse lung using 10413-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10413-1-AP (PLUNC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10413-1-AP

  IP result of anti-PLUNC (IP:10413-1-AP, 4ug; Detection:10413-1-AP 1:300) with mouse lung tissue lysate 3600ug.

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  IHC staining of mouse lung using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: mouse lung tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Product Information

10413-1-AP targets PLUNC in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLUNC fusion protein Ag0629
• Full Name: palate, lung and nasal epithelium associated
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012549
• Gene Symbol: PLUNC
• Gene ID (NCBI): 51297
• RRID: AB_2166093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLUNC, also named as LUNX, NASG and SPURT, belongs to the BPI/LBP/Plunc superfamily and Plunc family. PLUNC may be involved in the airway inflammatory response after exposure to irritants. It is associated with tumor progression. PLUNC plays a role in innate immune responses of the upper airways.

Publications

Species	Application	Title
mouse	IF	Sci Rep; Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis; Authors - Chen-Qian Zhao
mouse,human	WB,IHC,IF,ChIP	Cell Death Dis; USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24; Authors - Huai Liu
human,mouse	WB	Front Immunol; CC16 drives VLA-2-dependent SPLUNC1 expression; Authors - Natalie Iannuzo
human	WB	Med Oncol; SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells; Authors - Ling Tang