PLVAP Recombinant monoclonal antibody, PBS Only

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• PLVAP Recombinant monoclonal antibody, PBS Only (87712-1-PBS)

PLVAP Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 87712-1-PBS

Clone No.252917G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein, PV 1

• 

  WB analysis using 87712-1-RR (same clone as 87712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87712-1-RR (PLVAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87712-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 87712-1-RR (same clone as 87712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87712-1-RR (PLVAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87712-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using 87712-1-RR (same clone as 87712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87712-1-RR (PLVAP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87712-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87712-1-PBS targets PLVAP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5463

  Product name: Recombinant human PLVAP protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 49-442 aa of NM_031310.2

  Sequence: YGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG

• Full Name: plasmalemma vesicle associated protein
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_031310.2
• Gene Symbol: PLVAP
• Gene ID (NCBI): 83483
• RRID: AB_3745711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BX97
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Plasmalemma vesicle-associated protein (PLVAP) is an endothelial-associated protein that is involved in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, PLVAP has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry into soluble ags into lymph node conduits .(PMID: 36884119, PMID: 37810232)