PMF1 Polyclonal antibody

PMF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11190-2-AP

Synonyms

PMF-1, Polyamine-modulated factor 1, polyamine modulated factor 1, PMF 1

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Validation Data Gallery

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  WB analysis of Jurkat using 11190-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11190-2-AP (PMF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11190-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11190-2-AP (PMF1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11190-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11190-2-AP (PMF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11190-2-AP

  Immunofluorescent analysis of HepG2 cells using 11190-2-AP (PMF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11190-2-AP targets PMF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PMF1 fusion protein Ag1672
• Full Name: polyamine-modulated factor 1
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC033656
• Gene Symbol: PMF1
• Gene ID (NCBI): 11243
• RRID: AB_2064906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.