PMM1 Polyclonal antibody

PMM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11252-2-AP

Synonyms

phosphomannomutase 1, PMM 1, PMM1, PMMH 22, PMMH22, Sec53

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Validation Data Gallery

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  WB analysis of mouse brain using 11252-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11252-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11252-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11252-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11252-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11252-2-AP targets PMM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PMM1 fusion protein Ag1782
• Full Name: phosphomannomutase 1
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC016818
• Gene Symbol: PMM1
• Gene ID (NCBI): 5372
• RRID: AB_2166971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMM1, also named as PMMH22, belongs to the eukaryotic PMM family. It catalyzes the second step in the conversion of fructose-6P to GDP-mannoseis and is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, PMM1 may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain(PMID:18927083).

Publications

Species	Application	Title
mouse	WB	J Proteome Res; Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese.; Authors - Wegrzynowicz Michal M