PMVK Polyclonal antibody

PMVK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15674-1-AP

Synonyms

hPMK, HUMPMKI, phosphomevalonate kinase, PMK, PMKA, PMKASE, PMKI, PMVK



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15674-1-AP targets PMVK in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMVK fusion protein Ag8141
Full Name phosphomevalonate kinase
Calculated Molecular Weight 192 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC007694
Gene Symbol PMVK
Gene ID (NCBI) 10654
RRIDAB_2299910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PMVK antibody 15674-1-APDownload protocol
IHC protocol for PMVK antibody 15674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.

Authors - Jiuxiang Wang
humanWB

Sci Rep

MicroRNA-874 targets phosphomevalonate kinase and inhibits cancer cell growth via the mevalonate pathway

Authors - Alimasi Aersilan