PNCK Polyclonal antibody

PNCK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13712-1-AP

Synonyms

BSTK3, CaM KI beta, CaM kinase I beta, CaM kinase IB, CaMK1b, CaMKI beta, PNCK

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Validation Data Gallery

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  WB analysis using 13712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13712-1-AP (PNCK antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13712-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13712-1-AP (PNCK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 13712-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 13712-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13712-1-AP (PNCK antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IHC detected in: human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:9000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13712-1-AP targets PNCK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PNCK fusion protein Ag4650
• Full Name: pregnancy up-regulated non-ubiquitously expressed CaM kinase
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 38 kDa, 45 kDa
• GenBank Accession Number: BC033746
• Gene Symbol: PNCK
• Gene ID (NCBI): 139728
• RRID: AB_10642952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.