PNKP Polyclonal antibody

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  WB analysis of Jurkat using 13558-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13558-1-AP (PNKP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13558-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13558-1-AP (PNKP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13558-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13558-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PNKP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13558-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DNA 5 kinase/3 phosphatase, PNK, PNKP

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13558-1-AP targets PNKP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4421

  Product name: Recombinant human PNKP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 171-521 aa of BC033822

  Sequence: DLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG

• Full Name: polynucleotide kinase 3'-phosphatase
• Calculated Molecular Weight: 521 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC033822
• Gene Symbol: PNKP
• Gene ID (NCBI): 11284
• RRID: AB_2877958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96T60
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Signal; Macrophage-derived exosome miR-146a-5p modulates PNKP/DDOST/JAGN1 complex to regulate NETs formation in atherosclerosis.; Authors - Zhen Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Isabelle Cristine (Verified Customer) (01-14-2022)

This antibody works extremely well for detecting PNKP in HEK293 cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293

PNKP Antibody Western Blot validation (1:500 dilution) in HEK293 (Cat no:13558-1-AP)