PNMA1 Polyclonal antibody

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PNMA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 13631-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

37 kDa neuronal protein, MA1, paraneoplastic antigen MA1, PNMA1

• 

  WB analysis using 13631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13631-1-AP (PNMA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse testis using 13631-1-AP

  IP result of anti-PNMA1 (IP:13631-1-AP, 4ug; Detection:13631-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13631-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13631-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 13631-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 13631-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13631-1-AP (PNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 13631-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13631-1-AP (PNMA1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 13631-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13631-1-AP (PNMA1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PC-3 cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse brain tissue, mouse testis tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13631-1-AP targets PNMA1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4549

  Product name: Recombinant human PNMA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-353 aa of BC039577

  Sequence: MAMTLLEDWCRGMDVNSQRALLVWGIPVNCDEAEIEETLQAAMPQVSYRMLGRMFWREENAKAALLELTGAVDYAAIPREMPGKGGVWKVLFKPPTSDAEFLERLHLFLAREGWTVQDVARVLGFQNPTPTPGPEMPAEMLNYILDNVIQPLVESIWYKRLTLFSGRDIPGPGEETFDPWLEHTNEVLEEWQVSDVEKRRRLMESLRGPAADVIRILKSNNPAITTAECLKALEQVFGSVESSRDAQIKFLNTYQNPGEKLSAYVIRLEPLLQKVVEKGAIDKDNVNQARLEQVIAGANHSGAIRRQLWLTGAGEGPAPNLFQLLVQIREEEAKEEEEEAEATLLQLGLEGHF

• Full Name: paraneoplastic antigen MA1
• Calculated Molecular Weight: 353 aa, 40 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC039577
• Gene Symbol: PNMA1
• Gene ID (NCBI): 9240
• RRID: AB_2167615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8ND90
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The PNMA1 Polyclonal antibody (Cat# 13631-1-AP) has 6 citations published in Nature Aging, Scientific Reports, Biomedical Pharmacotherapy, International Journal of Clinical and Experimental Pathology, Research Square, and bioRxiv. The associated research fields span reproductive biology and aging, DNA repair and chemoresistance in retinoblastoma, and oncology including hepatocellular carcinoma and pancreatic ductal adenocarcinoma.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Aging; The retrotransposon-derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity; Authors - Thomas W P Wood
human	WB	Sci Rep; PNMA1 mediates melphalam chemoresistance in retinoblastoma via promoting DNA repair.; Authors - Longge Sun
human	WB	Biomed Pharmacother; PNMA1, regulated by miR-33a-5p, promotes proliferation and EMT in hepatocellular carcinoma by activating the Wnt/β-catenin pathway.; Authors - Pengpeng Liu ; KD Validated
human	WB,IHC	Int J Clin Exp Pathol; PNMA1 promotes cell growth in human pancreatic ductal adenocarcinoma.; Authors - Shu-Heng Jiang ; KD Validated
mouse	WB	bioRxiv; The retrotransposon - derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity; Authors - Thomas W P Wood ; KO Validated
human	WB	Res Sq; The Retrotransposon-Derived Capsid Genes PNMA1 and PNMA4 Maintain Reproductive Capacity; Authors - Luke Berchowitz