PNMA1 Monoclonal antibody

• 

  WB analysis of PC-3 using 68415-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 68415-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 68415-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 68415-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 68415-1-Ig (PNMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of PC-3 using 68415-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PNMA1 antibody (68415-1-Ig, Clone: 2D8C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

PNMA1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68415-1-Ig

Clone No.2D8C6

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Paraneoplastic antigen Ma1, MA1, 37 kDa neuronal protein, 2D8C6

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Tested Applications

• Positive WB detected in: PC-3 cells, T-47D cells, LNCaP cells, rat testis tissue
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68415-1-Ig targets PNMA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33143

  Product name: Recombinant human PNMA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-353 aa of BC039577

  Sequence: MAMTLLEDWCRGMDVNSQRALLVWGIPVNCDEAEIEETLQAAMPQVSYRMLGRMFWREENAKAALLELTGAVDYAAIPREMPGKGGVWKVLFKPPTSDAEFLERLHLFLAREGWTVQDVARVLGFQNPTPTPGPEMPAEMLNYILDNVIQPLVESIWYKRLTLFSGRDIPGPGEETFDPWLEHTNEVLEEWQVSDVEKRRRLMESLRGPAADVIRILKSNNPAITTAECLKALEQVFGSVESSRDAQIKFLNTYQNPGEKLSAYVIRLEPLLQKVVEKGAIDKDNVNQARLEQVIAGANHSGAIRRQLWLTGAGEGPAPNLFQLLVQIREEEAKEEEEEAEATLLQLGLEGHF

• Full Name: paraneoplastic antigen MA1
• Calculated Molecular Weight: 353 aa, 40 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC039577
• Gene Symbol: PNMA1
• Gene ID (NCBI): 9240
• RRID: AB_3085131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8ND90
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.