PNPLA7 Polyclonal antibody

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  IHC staining of mouse cerebellum using 27787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27787-1-AP (PNPLA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 27787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27787-1-AP (PNPLA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PNPLA7 Polyclonal Antibody for IHC, ELISA

Cat No. 27787-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

RP11 48C7.2, NTEL1, NTE R1, C9orf111

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Tested Applications

• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27787-1-AP targets PNPLA7 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PNPLA7 fusion protein Ag27184
• Full Name: patatin-like phospholipase domain containing 7
• Calculated Molecular Weight: 146 kDa
• GenBank Accession Number: NM_001098537
• Gene Symbol: PNPLA7
• Gene ID (NCBI): 375775
• RRID: AB_3669623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZV29
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PNPLA7 (Patatin-like phospholipase domain-containing protein 7), also termed NTE-related 1 (NTE-R1) or NRE, is a member of the PNPLAs family, which is conserved protein in mice, rats and humans. It serves key roles in triglyceride hydrolysis, energy metabolism, lipid droplet (LD), and in regulation of adipocyte differentiation. It is reported that PNPLA7 was down-regulated in HCC through hypermethylation of its promoter, which indicates that PNPLA7 may be a potential biomarker for HCC diagnosis (PMID: 16799181, 27347198).