Various lysates were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15552-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
human liver tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15552-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human heart tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15552-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 15552-1-AP
IP result of anti-PNPO (IP:15552-1-AP, 4ug; Detection:15552-1-AP 1:500) with MCF-7 cells lysate 3200 ug.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
MCF-7 cells, human brain tissue, human heart tissue, human liver tissue, mouse brain tissue, rat brain tissue
Positive IP detected in
MCF-7 cells
Positive IHC detected in
human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Hela cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15552-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15552-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15552-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver using 15552-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15552-1-AP (PNPO antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 15552-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15552-1-AP (PNPO antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of MCF-7 using 15552-1-AP
IP result of anti-PNPO (IP:15552-1-AP, 4ug; Detection:15552-1-AP 1:500) with MCF-7 cells lysate 3200 ug.
IF/ICC Figures
IF Staining of HeLa using 15552-1-AP
Immunofluorescent analysis of Hela cells, using PNPO antibody 15552-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
PNPO Polyclonal antibody
Catalog Number
15552-1-AP
Citations
5
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
