Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human spleen tissue, human gliomas tissue, human skin tissue, human stomach cancer tissue, human lung tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14487-1-AP targets PNPT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5888 Product name: Recombinant human PNPT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 427-783 aa of BC053660 Sequence: YEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ Predict reactive species |
| Full Name | polyribonucleotide nucleotidyltransferase 1 |
| Calculated Molecular Weight | 86 kDa |
| Observed Molecular Weight | 86 kDa |
| GenBank Accession Number | BC053660 |
| Gene Symbol | PNPT1 |
| Gene ID (NCBI) | 87178 |
| RRID | AB_2165820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TCS8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PNPT1 antibody 14487-1-AP | Download protocol |
| IHC protocol for PNPT1 antibody 14487-1-AP | Download protocol |
| IP protocol for PNPT1 antibody 14487-1-AP | Download protocol |
| WB protocol for PNPT1 antibody 14487-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PNPT1 antibody (Cat# 14487-1-AP) has 18 citations across 15 journals, including Immunity, Nature Communications, Molecular Cell, PNAS, EMBO Journal, Cell Reports, PLoS Genetics, and American Journal of Human Genetics. Research fields span pyroptosis/PANoptosis, inflammasome activation, mitochondrial RNA metabolism, hereditary hearing loss, acute myeloid leukemia, viral propagation, neurodegeneration, and 22q11.2 microdeletion genetics.
| Species | Application | Title |
|---|---|---|
Immunity Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis
| ||
Cell Mol Immunol Pnpt1 mediates NLRP3 inflammasome activation by MAVS and metabolic reprogramming in macrophages
| ||
Nat Commun Mitochondrial RNA degradation regulates differentiation, stemness, and immune sensitivity in acute myeloid leukemia.
| ||
Nat Commun Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1 | ||
Proc Natl Acad Sci U S A EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells. |
Reviews
What customers say
One customer rated this antibody 5 out of 5 stars, noting it works well for both Western Blot and Immunofluorescence. The review was posted in January 2025 and used lot 00005700. Overall, the feedback is positive, confirming reliable performance across two common applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (01-31-2025) | good for WB and IF
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