PNPT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PNPT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14487-1-AP

Synonyms

PNPase old 35, PNPase 1, OLD35, old 35, EC:2.7.7.8

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Validation Data Gallery

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  WB analysis using 14487-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14487-1-AP (PNPT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14487-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14487-1-AP (PNPT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14487-1-AP

  IP result of anti-PNPT1 (IP:14487-1-AP, 4ug; Detection:14487-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14487-1-AP (PNPT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14487-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14487-1-AP (PNPT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, HeLa cells, Jurkat cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human skin tissue, human spleen tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

14487-1-AP targets PNPT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PNPT1 fusion protein Ag5888
• Full Name: polyribonucleotide nucleotidyltransferase 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC053660
• Gene Symbol: PNPT1
• Gene ID (NCBI): 87178
• RRID: AB_2165820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.

Publications

Species	Application	Title
human	WB	Immunity; Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis; Authors - Rui Miao ; KD Validated
mouse	WB,IHC	Am J Hum Genet; A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.; Authors - von Ameln Simon S ; KO Validated
human,mouse	WB,IF	Int J Antimicrob Agents; Pan-viral propagation blockade by inhibiting host cell PNPT1; Authors - Shuang Qu
mouse	IF	Proc Natl Acad Sci U S A; EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells.; Authors - Tina Pangršič
mouse	WB	EMBO J; CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.; Authors - Karolina Szczepanowska
human	WB	PLoS Genet; RNA binding protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer.; Authors - Mrinmoyee Majumder