PNPT1 Monoclonal antibody, PBS Only

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  WB analysis using 68309-1-Ig (same clone as 68309-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68309-1-Ig (PNPT1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPT1 antibody (68309-1-Ig, Clone: 3F2A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68309-1-Ig (same clone as 68309-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.2 μg PNPT1 Monoclonal antibody (68309-1-Ig, Clone:3F2A1, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone:1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  FC experiment of LNCaP using 68309-1-Ig (same clone as 68309-1-PBS)

  1x10^6 LNCaP cells were intracellularly stained with 0.2 μg PNPT1 Monoclonal antibody (68309-1-Ig, Clone:3F2A1, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone:1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

PNPT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 68309-1-PBS

Clone No.3F2A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

3'-5' RNA exonuclease OLD35, 3F2A1, EC:2.7.7.8, old 35, OLD35

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Product Information

68309-1-PBS targets PNPT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6290

  Product name: Recombinant human PNPT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 427-783 aa of BC053660

  Sequence: YEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ

• Full Name: polyribonucleotide nucleotidyltransferase 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC053660
• Gene Symbol: PNPT1
• Gene ID (NCBI): 87178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8TCS8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.