POFUT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

POFUT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 14929-1-AP

Synonyms

O FucT 1, O Fuc T, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, FUT12

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Validation Data Gallery

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  WB analysis of HCT 116 using 14929-1-AP

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 14929-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14929-1-AP

  IP result of anti-POFUT1 (IP:14929-1-AP, 4ug; Detection:14929-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 14929-1-AP

  Immunofluorescent analysis of A431 cells, using POFUT1 antibody 14929-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HUVEC using 14929-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using POFUT1 antibody (14929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HCT 116 cells, HUVEC cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Product Information

14929-1-AP targets POFUT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POFUT1 fusion protein Ag6710
• Full Name: protein O-fucosyltransferase 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC000582
• Gene Symbol: POFUT1
• Gene ID (NCBI): 23509
• RRID: AB_2166123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.

Publications

Species	Application	Title
mouse	WB	Cell; In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.; Authors - Hongling Huang ; KO Validated
human	WB,IHC,IF	EBioMedicine; PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.; Authors - Daojiang Li ; KD Validated
human	WB	EMBO Mol Med; A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder; Authors - Ren e G Feichtinger
human	WB,IHC,IF	Cell Death Dis; POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.; Authors - Yuheng Du ; KD Validated
human	IHC, IF	Cell Death Dis; LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.; Authors - S Liu
human	WB	Cell Prolif; Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA.; Authors - Xinyuan Cui