POGK Polyclonal antibody

POGK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24888-1-AP

Synonyms

BASS2, KIAA1513, KIAA15131, LST003, POGK, SLTP003

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Validation Data Gallery

• 

  WB analysis of A549 using 24888-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24888-1-AP (POGK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24888-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24888-1-AP (POGK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 24888-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 24888-1-AP (POGK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, human testis tissue, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24888-1-AP is POGK Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POGK fusion protein Ag21681
• Full Name: pogo transposable element with KRAB domain
• Calculated Molecular Weight: 609 aa, 69 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC131624
• Gene Symbol: POGK
• Gene ID (NCBI): 57645
• RRID: AB_2879779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POGK also named as pogo transposable element with KRAB domain is a 609 amino acid protein, which contains one DDE domain, 1 KRAB domain and one HTH CENPB-type DNA binding domain. POGK belongs to DNA-mediated transposons family and may involve in the regulation of gene transcription.