POLD2 Polyclonal antibody

POLD2 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10288-1-AP

Synonyms

DNA polymerase delta subunit 2, POLD2

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Validation Data Gallery

• 

  WB analysis of HepG2 using 10288-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10288-1-AP (POLD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10288-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10288-1-AP (POLD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 10288-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10288-1-AP (POLD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 10288-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10288-1-AP (POLD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, mouse pancreas tissue, SGC-7901 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

10288-1-AP targets POLD2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLD2 fusion protein Ag0270
• Full Name: polymerase (DNA directed), delta 2, regulatory subunit 50kDa
• Calculated molecular weight: 51 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC000459
• Gene symbol: POLD2
• Gene ID (NCBI): 5425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.

Publications

Species	Application	Title
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB	Nucleic Acids Res; Regulation of error-prone translesion synthesis by Spartan/C1orf124.; Authors - Kim Myoung Shin MS