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POLD2 Polyclonal antibody, PBS Only (29765-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 29765-1-AP (same clone as 29765-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.
WB analysis using 29765-1-AP (same clone as 29765-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.
WB analysis using 29765-1-AP (same clone as 29765-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 29765-1-AP (same clone as 29765-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 29765-1-AP (same clone as 29765-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 29765-1-AP (same clone as 29765-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD2 antibody (29765-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

POLD2 Polyclonal antibody, PBS Only

Name: POLD2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 29765-1-PBS

POLD2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29765-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DNA polymerase delta subunit 2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

29765-1-PBS targets POLD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag31521 Product name: Recombinant human POLD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 40-195 aa of BC000459 Sequence: RLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRF Predict reactive species
Full Name	polymerase (DNA directed), delta 2, regulatory subunit 50kDa
Calculated Molecular Weight	51 kDa
Observed Molecular Weight	48-51 kDa
GenBank Accession Number	BC000459
Gene Symbol	POLD2
Gene ID (NCBI)	5425
RRID	AB_2918344
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49005
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.