POLDIP2 Polyclonal antibody

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• POLDIP2 Polyclonal antibody (15080-1-AP)

POLDIP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15080-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2

• 

  WB analysis using 15080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15080-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 15080-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 15080-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 15080-1-AP

  IP result of anti-POLDIP2 (IP:15080-1-AP, 4ug; Detection:15080-1-AP 1:1000) with HeLa cells lysate 1600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 15080-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15080-1-AP (POLDIP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 15080-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15080-1-AP (POLDIP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 15080-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLDIP2 antibody (15080-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15080-1-AP targets POLDIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7154

  Product name: Recombinant human POLDIP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-368 aa of BC000655

  Sequence: MAACTARRALAVGSRWWSRSLTGARWPRPLCAAAGAGAFSPASTTTTRRHLSSRNRPEGKVLETVGVFEVPKQNGKYETGQLFLHSIFGYRGVVLFPWQARLYDRDVASAAPEKAENPAGHGSKEVKGKTHTYYQVLIDARDCPHISQRSQTEAVTFLANHDDSRALYAIPGLDYVSHEDILPYTSTDQVPIQHELFERFLLYDQTKAPPFVARETLRAWQEKNHPWLELSDVHRETTENIRVTVIPFYMGMREAQNSHVYWWRYCIRLENLDSDVVQLRERHWRIFSLSGTLETVRGRGVVGREPVLSKEQPAFQYSSHVSLQASSGHMWGTFRFERPDGSHFDVRIPPFSLESNKDEKTPPSGLHW

• Full Name: polymerase (DNA-directed), delta interacting protein 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000655
• Gene Symbol: POLDIP2
• Gene ID (NCBI): 26073
• RRID: AB_2166599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2S7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).

Publications

What published studies show

The POLDIP2 Polyclonal antibody (Cat# 15080-1-AP) has 6 citations published in Advanced Science, PNAS, EMBO Journal, Life Sciences, Cells, and Cell Cycle. Research fields span skeletal homeostasis and osteoporosis, DNA replication and damage bypass, mitochondrial DNA elimination during spermatogenesis, diabetic retinopathy and mitophagy, mitochondrial protein dynamics, and UV-induced alternative splicing. Applications cited include WB and IF.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Adv Sci (Weinh); Amuc_1473 Links Gut Microbes to Skeletal Homeostasis and Counteracts Multifactorial Osteoporosis.; Authors - Shan-Shan Rao
mouse	WB	Proc Natl Acad Sci U S A; DNA polymerase δ-interacting protein 2 is a processivity factor for DNA polymerase λ during 8-oxo-7,8-dihydroguanine bypass.; Authors - Maga Giovanni G
	WB	EMBO J; Poldip2 promotes mtDNA elimination during Drosophila spermatogenesis to ensure maternal inheritance; Authors - Ziming Wang
mouse,rat	WB	Life Sci; Poldip2 Aggravates inflammation in diabetic retinopathy by impairing mitophagy via the AMPK/ULK1/Pink1 pathway; Authors - Siyu Lin
mouse,human	WB	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key
human	IF	Cell Cycle; PDIP38 is translocated to the spliceosomes/nuclear speckles in response to UV-induced DNA damage and is required for UV-induced alternative splicing of MDM2.; Authors - Agnes Wong