POLDIP2 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86232-3-RR (same clone as 86232-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86232-3-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 86232-3-RR (same clone as 86232-3-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86232-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 86232-3-RR (same clone as 86232-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLDIP2 antibody (86232-3-RR, Clone: 250999B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86232-3-PBS in a different storage buffer formulation.

POLDIP2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86232-3-PBS

Clone No.250999B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2

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Product Information

86232-3-PBS targets POLDIP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7154

  Product name: Recombinant human POLDIP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-368 aa of BC000655

  Sequence: MAACTARRALAVGSRWWSRSLTGARWPRPLCAAAGAGAFSPASTTTTRRHLSSRNRPEGKVLETVGVFEVPKQNGKYETGQLFLHSIFGYRGVVLFPWQARLYDRDVASAAPEKAENPAGHGSKEVKGKTHTYYQVLIDARDCPHISQRSQTEAVTFLANHDDSRALYAIPGLDYVSHEDILPYTSTDQVPIQHELFERFLLYDQTKAPPFVARETLRAWQEKNHPWLELSDVHRETTENIRVTVIPFYMGMREAQNSHVYWWRYCIRLENLDSDVVQLRERHWRIFSLSGTLETVRGRGVVGREPVLSKEQPAFQYSSHVSLQASSGHMWGTFRFERPDGSHFDVRIPPFSLESNKDEKTPPSGLHW

• Full Name: polymerase (DNA-directed), delta interacting protein 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC000655
• Gene Symbol: POLDIP2
• Gene ID (NCBI): 26073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y2S7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).