POLE3 Polyclonal antibody

POLE3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15278-1-AP

Synonyms

Arsenic transactivated protein, AsTP, CHARAC17, CHRAC 17, CHRAC17, DNA polymerase II subunit 3, HuCHRAC17, p17, POLE3, YBL1



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15278-1-AP targets POLE3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLE3 fusion protein Ag7396
Full Name polymerase (DNA directed), epsilon 3 (p17 subunit)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC003166
Gene symbol POLE3
Gene ID (NCBI) 54107
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLE3 antibody 15278-1-APDownload protocol
IHC protocol for POLE3 antibody 15278-1-APDownload protocol
IF protocol for POLE3 antibody 15278-1-APDownload protocol
IP protocol for POLE3 antibody 15278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biochem Cell Biol

SNORA42 enhances prostate cancer cell viability, migration and EMT and is correlated with prostate cancer poor prognosis.

Authors - Chuanyou Yi