POLI Polyclonal antibody

• Featured Product
• KD/KO Validated

POLI Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 13635-1-AP

Synonyms

DNA polymerase iota, Eta2, POLI, polymerase (DNA directed) iota, RAD30 homolog B, RAD30B, RAD3OB

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Validation Data Gallery

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  WB analysis using 13635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13635-1-AP (POLI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13635-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13635-1-AP (POLI antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13635-1-AP

  IP result of anti-POLI (IP:13635-1-AP, 4ug; Detection:13635-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 13635-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13635-1-AP (POLI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13635-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13635-1-AP (POLI antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human heart tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Product Information

13635-1-AP targets POLI in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, mosquito
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLI fusion protein Ag4563
• Full Name: polymerase (DNA directed) iota
• Calculated Molecular Weight: 715 aa, 80 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC032662
• Gene Symbol: POLI
• Gene ID (NCBI): 11201
• RRID: AB_2167009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian Pol ι owns an unusual combination of properties: it is stimulated by Mn2+ ions, can bypass some DNA lesions and misincorporates "G" opposite template "T" more frequently than incorporates the correct "A." [PMID:21304950] It also was shown to possess dRP lyase activity. In addition, Pol ι is much more efficient in the presence of Mn2+ in comparison to Mg2+. [PMID:17609217]. It has been proposed that POLI involves in immunoglobulin somatic hypermutation (SHM), bypass of deaminated cytosines, several adducts of the purine bases. DNA strand crosslinks and is involved in DNA repair under oxidative stress[PMID:15199127].

Publications

Species	Application	Title
human	WB,IHC,IF	Cell Death Dis; DNA polymerase iota promotes EMT and metastasis of esophageal squamous cell carcinoma by interacting with USP7 to stabilize HIF-1α; Authors - Aidi Gao
mouse	WB	Oncogene; The error-prone DNA polymerase ι provides quantitative resistance to lung tumorigenesis and mutagenesis in mice.; Authors - M Iguchi
human	WB	Front Oncol; DNA Polymerase Iota Promotes Esophageal Squamous Cell Carcinoma Proliferation Through Erk-OGT-Induced G6PD Overactivation.; Authors - Zhenzi Su ; KD Validated
human	WB,IHC	Cancer Sci; Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma.; Authors - Chao He
mosquito	IF	Insect Mol Biol; Diverse cellular morphologies during lumen maturation in Anopheles gambiae larval salivary glands.; Authors - M Chiu
human	IHC, IF	Int J Clin Exp Pathol; Elevated DNA polymerase iota (Poli) is involved in the acquisition of aggressive phenotypes of human esophageal squamous cell cancer.; Authors - Haoyao Sun