Tested Applications
| Positive WB detected in | HeLa cells, Saos-2 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14671-1-AP targets POLL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6337 Product name: Recombinant human POLL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC068529 Sequence: MDPRGILKAFPKRQKIHADASSKVLAKIPRREEGEEAEEWLSSLRAHVVRTGIGRARAELFEKQIVQHGGQLCPAQGPGVTHIVVDEGMDYERALRLLRLPQLPPGAQLVKSAWLSLCLQERRLVDVAGFSIFIPSRYLDHPQPSKAEQDASIPPGTHEALLQTALSPPPPPTRPVSPPQKAKEAPNTQAQPISDDEASDGEETQVSAADLEALISGHYPTSLEGDCEPSPAPAVLDKWVCAQPSSQKATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQGACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQM Predict reactive species |
| Full Name | polymerase (DNA directed), lambda |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 63-70 kDa |
| GenBank Accession Number | BC068529 |
| Gene Symbol | POLL |
| Gene ID (NCBI) | 27343 |
| RRID | AB_3741856 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGP5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
POLL (DNA polymerase lambda) functions in DNA repair (PMID: 11457865, PMID: 19806195, PMID: 20693240, PMID: 30250067). POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, indicating that POLL participates in short- and long-patch base excision repair. POLL is involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PMID: 11457865, PMID: 19806195).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POLL antibody 14671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The POLL polyclonal antibody (Cat# 14671-1-AP) has 1 total citation. It was cited in Proceedings of the National Academy of Sciences of the United States of America (PNAS), a high-impact journal (IF 9.5). The cited study investigated DNA polymerase λ's role as a processivity factor during 8-oxo-7,8-dihydroguanine bypass, placing the research in the field of DNA repair, specifically base excision repair. The application used was Western blotting with mouse samples.
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A DNA polymerase δ-interacting protein 2 is a processivity factor for DNA polymerase λ during 8-oxo-7,8-dihydroguanine bypass. |
