POLQ Polyclonal antibody, PBS Only

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• POLQ Polyclonal antibody, PBS Only (28590-1-PBS)

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  WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

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  WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 28590-1-AP (same clone as 28590-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 28590-1-AP (same clone as 28590-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

POLQ Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 28590-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

POLH, EC:2.7.7.49, DNA polymerase theta, DNA polymerase eta

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Product Information

28590-1-PBS targets POLQ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29643

  Product name: Recombinant human POLQ protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 2342-2590 aa of NM_199420

  Sequence: LSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

• Full Name: polymerase (DNA directed), theta
• Calculated Molecular Weight: 290 kDa
• Observed Molecular Weight: 250~290 kDa, 197 kDa
• GenBank Accession Number: NM_199420
• Gene Symbol: POLQ
• Gene ID (NCBI): 10721
• RRID: AB_3086067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75417
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs). The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. Accumulating evidence showed that POLQ participates in the development and progression of human cancers because of its critical functions in the repair of genomic double-strand breaks (PMID: 34517891, 27264557). The POLQ cDNA encodes a polypeptide of 2592 amino acids with a predicted mass of 290 kDa, but POLQ is readily proteolyzed with smaller bands at approximately 70 kDa and 110 kDa (PMID: 17334634, 14576298).