POLR1C Polyclonal antibody

POLR1C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 15923-1-AP

Synonyms

AC40, POLR1C, POLR1E, RPA39, RPA40, RPA5, RPAC1, RPC40

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Validation Data Gallery

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  WB analysis using 15923-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15923-1-AP (POLR1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15923-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15923-1-AP (POLR1C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 15923-1-AP

  IP result of anti-POLR1C (IP:15923-1-AP, 4ug; Detection:15923-1-AP 1:800) with NIH/3T3 cells lysate 1600 ug.

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  IHC staining of human ovary using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HEK-293 cells, Caco-2 cells, HeLa cells, HepG2 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human ovary tissue, mouse liver tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human liver tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15923-1-AP targets POLR1C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR1C fusion protein Ag8746
• Full Name: polymerase (RNA) I polypeptide C, 30kDa
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC008863
• Gene Symbol: POLR1C
• Gene ID (NCBI): 9533
• RRID: AB_2167328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR1C is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).

Publications

Species	Application	Title
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Krishnendu (Verified Customer) (02-27-2020)

Nice antibody. Got a specific band.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T