POLR2A Polyclonal antibody

POLR2A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 20655-1-AP

Synonyms

hRPB220, hsRPB1, Pol II, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

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Validation Data Gallery

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  WB analysis of HeLa using 20655-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20655-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20655-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 20655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20655-1-AP (POLR2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 20655-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20655-1-AP (POLR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, NIH/3T3 cells
• Positive IHC detected in: human pancreas cancer tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

20655-1-AP targets POLR2A in WB, IF, IHC, ELISA applications and shows reactivity with human, monkey, mouse samples.

• Tested Reactivity: human, monkey, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Calculated Molecular Weight: 217 kDa
• Observed Molecular Weight: 210-250 kDa
• GenBank Accession Number: NM_000937
• Gene Symbol: POLR2A
• Gene ID (NCBI): 5430
• RRID: AB_2878716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Brahma-Related Gene 1 Deficiency in Endothelial Cells Ameliorates Vascular Inflammatory Responses in Mice.; Authors - Yuanyuan Zhang
human	WB,IF	J Exp Clin Cancer Res; LncMAPK6 drives MAPK6 expression and liver TIC self-renewal.; Authors - Guanqun Huang
human	WB	Cell Death Discov; Cezanne promoted autophagy through PIK3C3 stabilization and PIK3C2A transcription in lung adenocarcinoma; Authors - Yadong Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marta (Verified Customer) (02-28-2022)

Method: dot blot

• Applications: Other
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: glioma

POLR2A Antibody Other validation (1:1000 dilution) in glioma (Cat no:20655-1-AP)