Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20655-1-AP targets POLR2A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, monkey samples.
| Tested Reactivity | human, mouse, monkey |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | polymerase (RNA) II (DNA directed) polypeptide A, 220kDa |
| Calculated Molecular Weight | 217 kDa |
| Observed Molecular Weight | 210-250 kDa |
| GenBank Accession Number | NM_000937 |
| Gene Symbol | POLR2A |
| Gene ID (NCBI) | 5430 |
| RRID | AB_2878716 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24928 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for POLR2A antibody 20655-1-AP | Download protocol |
| IP protocol for POLR2A antibody 20655-1-AP | Download protocol |
| WB protocol for POLR2A antibody 20655-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The POLR2A Polyclonal antibody (Cat# 20655-1-AP) has 7 total citations. These appear in journals including Nature Communications, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cell Death Discovery, Cell Reports, Frontiers in Cell and Developmental Biology, and Neuropharmacology. Research fields span metabolic regulation, liver cancer stem cell self-renewal, NAD⁺ metabolism and early embryonic development, autophagy in lung adenocarcinoma, colorectal cancer initiation, vascular inflammation, and chronic pain with neurotransmitter imbalance.
| Species | Application | Title |
|---|---|---|
Nat Commun Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1 | ||
Adv Sci (Weinh) NAD(+) Metabolism Licenses Zygotic Genome Activation via PARP7-Mediated ADP-Ribosylation of UHRF1 in Mouse Early Embryos. | ||
Cell Death Discov Cezanne promoted autophagy through PIK3C3 stabilization and PIK3C2A transcription in lung adenocarcinoma
| ||
Cell Rep NEIL1 drives the initiation of colorectal cancer through transcriptional regulation of COL17A1 | ||
Front Cell Dev Biol Brahma-Related Gene 1 Deficiency in Endothelial Cells Ameliorates Vascular Inflammatory Responses in Mice. |
Reviews
What customers say
The single review for 20655-1-AP is from Marta Grabiec (February 2022), who rated the product 5 stars. She used it in a dot blot application at a 1:1000 dilution on glioma tissue. The review includes an image of her results. Overall, the feedback is positive, indicating the antibody performed well for detecting its target in glioma samples under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marta (Verified Customer) (02-28-2022) | Method: dot blot
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